CRISPR Lab

Our Programmes

Knockout! A CRISPR/CAS Gene Targeting Lab

CRISPR/Cas 9 became an international science buzzword way before two of the pioneers of the technology (Emmanuelle Charpentier and Jennifer Doudna) were awarded the Nobel Prize in 2020 for their contribution to science. Come and join us in this newly designed miniPCR lesson to experience and understand this revolutionary gene-editing technology.

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Program Details

This lab gives advanced students the opportunity to perform Cas9-mediated gene targeting. Scientists use this CRISPR/Cas system to alter DNA in living cells. Students will get to experience being a genetic engineer themselves and use the CRISPR/Cas system to disrupt a gene in bacteria.

Learning Outcomes

  • Understand genome editing
  • Explain how CRISPR/Cas9 is used to target specific DNA sequences.
  • Culturing bacteria

Total Estimated Time Period

180 mins

Difficulty Level

Advanced

Equipment Used

  • Micro-pipettes
  • miniPCR blueGel™ gel electrophoresis tank (part 2)
  • miniPCR mini8 thermocycler (part 2)